Malate Dehydrogenase(MDH)

Cytoplasmic recombinant MDH produced in E. coli MDH is fused to 6 histidine amino acids, His-tag, at N-terminus.

Applications Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Preparation and Storage For long term storage, store at -20~-70 °C Avoiding freeze/thaw cycles. Michaelis constants NADH = 20.6 μM or 0.02 mM Application

This enzyme is useful for enzymatic determination of L- malate and of glutamate oxaloacetate transaminase in clinical analysis.

Molecular weight: ca. 36,000 Optimum pH: 7.5 pH stability: 5.5 - 11.0 Thermal stability: No detectable decrease in activity up to 60 °C. Michaelis constants: (150mM PBS buffer, pH 7.5, at 25 °C)

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