Recombinant Streptavidin Protein

E.coli (Extinction coefficient = 32. 1%. 280 nm) Purity: >95% by SDS-page Purification: Ni column Liquid; PBS (pH 7.2), 10% Glycerol 1.0 mg/ml (lot specific) Molecular Weight Approx. 54,000 Da pH-stability pH 5.0 – 9.0

The protein is free of carbohydrate. The four subunits consisting of molecular weight of approx. 16,000 Da, each containing a single biotin-binding site. Each subunit has six tyrosine residues.

Streptavidin can be used for coating of solid phases (e.g., micro plates, beads etc.) as well as for use with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

For long term storage, store at -20~-70 °C Avoiding freeze/thaw cycles.

Flow Cytometry: 5-10 μg/ml ICC: 5-10 μg/ml IHC-P: 5-10 μg/ml or IHC-F: 5-10 μg/ml Optimal working dilutions must be determined by end user.

STORAGE: The product is stable f at 2-8°C as an undiluted liquid.

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